Home/ CSDM15 - Chromosome Structure Determination using Modelling and Hi-C Data
CSDM15 - Chromosome Structure Determination using Modelling and Hi-C Data
Tue, 2015-11-24 to Fri, 2015-11-27
The sequence of a genome alone does not carry enough information to fully understand how genomic processes are carried out in the cell nucleus; to achieve this, the knowledge of the three-dimensional (3D) architecture of a genome is necessary. Advances in genomic technologies and the development of new analytical methods, such as 3C-based methods, have allowed getting insights at unprecedented resolution into how the genome is organized. Recently, it has been shown that chromatin is organized in Topologically Associating Domains (TADs), large interaction domains that appear to be conserved among different cell types. In this course, participants will learn to use TADbit, a software for the analysis and modeling of Hi-C data.
The course will cover all the aspect of modeling with Hi-C data:
Generation of Hi-C contact matrices from raw data (that is, Hi-C specific quality check of the reads, mapping, filtering and normalization)
Analysis of Hi-C matrices (Hi-C map visualization, TAD identification, experiment comparison, and TAD selection)
3D modeling and analysis of TADs
The 3D module of TADbit is based on a computational module of the Integrative Modeling Platform (IMP, http://www.integrativemodeling.org) that uses chromosome conformation capture data to determine the 3D architecture of genomic domains and entire genomes at unprecedented resolutions.
Participants can bring-in specific biological questions and/or their on data to work on during the course.
At the end of the course, participants will be familiar with the TADbit software, enabling them to fully analyze and model Hi-C data.