Informatics for RNA-seq Analysis (2014)

Mon, 2014-06-02 to Tue, 2014-06-03
High-throughput sequencing of RNA libraries (RNA-seq) has become increasingly common and largely supplanted gene microarrays for transcriptome profiling. When processed appropriately, RNA-seq data has the potential to provide a considerably more detailed view of the transcriptome. The CBW has developed a 2-day course providing an introduction to RNA-seq data analysis followed by integrated tutorials demonstrating the use of popular RNA-seq analysis packages. The tutorials are designed as self contained units that include example data (Illumina paired-end RNA-seq data) and detailed instructions for installation of all required bioinformatics tools (TopHat, Cufflinks, etc.). Participants will gain practical experience and skills to be able to: 1) Align RNA-seq data to a reference genome 2) Estimate known gene and transcript expression 3) Perform differential expression analysis 4) Discover novel isoforms 5) Visualize and summarize the output of RNA-seq analyses
Montreal, QC
$500 CAD