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Flow Cytometry 2013 Module 3 – Preprocessing and Quality Assurance of FCM Data

  • Preprocessing

    • Removing margin events
    • Data transformation: log vs. biexponential
    • Data normalization
  • Quality Assurance

    • Overview of quality assurance concepts: total raw/viable cell count, margin event count, outlier detection based on density of common parameters
    • Building quality assurance objects using flowQ and generating summary HTML reports

Audience

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