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Flow Cytometry 2013 Module 4 – 1D Static gating

  • How to create a constant gate for the whole data set for one channel by first creating a sample representative of the whole data set
  • How to apply a static gate to all samples and count the proportions of cells lying on either side of the gate
  • Visualizing the proportions using a density plot, histogram, bean/violin plot
  • Automating a sequential gating strategy in R to obtain cell proportions for a desired phenotype

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