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Flow Cytometry 2013 Module 5 – 1D Dynamic gating

  • Using quantiles to set a gate based on a negative control
  • Using median and standard deviation to set a gate based on a negative control
  • Exploring rangeGate: an automated way to set a 1D gate for each sample individually
  • Recording proportions of cells in subpopulations of interest by creating a spreadsheet fi le
  • Recording a visual record of the gating results by creating JPEG image fi les

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